Inactivation of Lactobacillus leichmannii Ribonucleotide Reductase by 2′,2′-Difluoro-2′-deoxycytidine 5′-Triphosphate: Covalent Modification
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چکیده
منابع مشابه
Inactivation of Lactobacillus leichmannii ribonucleotide reductase by F2CTP: covalent modification
Ribonucleotide reductase (RNR) from Lactobacillus leichmannii, a 76 kDa monomer using adenosylcobalamin (AdoCbl) as a cofactor, catalyzes the conversion of nucleoside triphosphates to deoxynucleotides and is rapidly (<30 sec) inactivated by one equivalent (eq.) of 2′,2′-difluoro-2′deoxycytidine 5′-triphosphate (F2CTP). [1′-3H] and [5-H]-F2CTP were synthesized and used independently to inactivat...
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Ribonucleotide reductase (RNR, 76 kDa) from Lactobacillus leichmannii is a class II RNR that requires adenosylcobalamin (AdoCbl) as a cofactor. It catalyzes the conversion on nucleoside triphosphates to deoxynucleotides and is 100% inactivated by one equivalent (eq.) of 2′,2′difluoro-2′-deoxycytidine 5′-triphosphate (F2CTP) in <2 min. Sephadex G50 chromatography of the inactivation reaction for...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2010
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi902132u